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sex chromosome, as in some grasshoppers and beetles; females are usually XX and males X0. And in the compound chromosome case, several X and Y chromosomes combine to determine sex, as in bedbugs and some beetles. We need to emphasize that the chromosomes themselves do not determine sex, but the genes they carry do. In general, the genotype determines the type of gonad, which then determines the phenotype of the organism through male or female hormonal production.
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The XY situation occurs in human beings, in which females have forty-six chromosomes arranged in twentythree homologous, homomorphic pairs Males, with the same number of chromosomes, have twenty-two homomorphic pairs and one heteromorphic pair, the XY pair ( g 51) During meiosis, females produce gametes that contain only the X chromosome, whereas males produce two kinds of gametes, X- and Y-bearing ( g 52) For this reason, females are referred to as homogametic and males as heterogametic As you can see from gure 52, in people, fertilization has an equal chance of producing either male or female offspring In Drosophila, the system is the same, but the Y chromosome is almost 20% larger than the X chromosome ( g 53).
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Since both human and Drosophila females normally have two X chromosomes, and males have an X and a Y chromosome, it seems impossible to know whether maleness is determined by the presence of a Y chromosome or the absence of a second X chromosome One way to resolve this problem would be to isolate individuals with odd numbers of chromosomes In chapter 8, we examine the causes and outcomes of anomalous chromosome numbers Here, we consider two facts from that chapter First, in rare instances, individuals form, although they are not necessarily viable, with extra sets of chromosomes These individuals are referred to as polyploids (triploids with 3n, tetraploids with 4n, etc) Second, also infrequently, individuals form that have more or fewer than the normal number of any one chromosome.
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These aneuploids usually come about when a pair of chromosomes fails to separate properly during meiosis, an occurrence called nondisjunction The existence of polyploid and aneuploid individuals makes it possible to test whether the Y chromosome is male determining For example, a person or a fruit y that has all the proper nonsex chromosomes, or autosomes (forty-four in human beings, six in Drosophila), but only a single X without a Y would answer our question If the Y were absolutely male determining, then this X0 individual should be female However, if the sex-determining mechanism is a result of the number of X chromosomes, this individual should be a male As it turns out, an X0 individual is a Drosophila male and a human female..
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Each area of genetics has its own particular techniques of study. Often the development of a new technique, or an improvement in a technique, has opened up major new avenues of research. As our technology has improved over the years, geneticists and other scientists have been able to explore at lower and lower levels of biological organization. Gregor Mendel, the father of genetics, did simple breeding studies of plants in a garden at his monastery in Austria in the middle of the nineteenth century. Today, with modern biochemical and biophysical techniques, it has become routine to determine the sequence of nucleotides (molecular subunits of DNA and RNA) that make up any particular gene. In fact, one of the most ambitious projects ever carried out in genetics is the mapping of the human genome, all 3.3 billion nucleotides that make up our genes. Only recently was the technology available to complete a project of this magnitude.
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