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2.3.5. Chemical-Based Inactivation of MEF Cells MEFs (p0 p4) are rapidly dividing cells and thus need to be mitotically inactivated before use as a feeder layer to prevent MEF overgrowth. Inactivation of the dividing cells can be brought about through irradiation or chemical blockade, the most commonly used being mitomycin C, which is a simple and reliable method of preparing MEFs for routine hES cell culture. Safety note: Care must be taken when handling mitomycin C as it is genotoxic. Gloves must always be worn, and the compound should only be handled in a Class II laminar ow hood (microbiological safety cabinet). For inactivation of cells for hES cell propagation, a 75-cm2 ask of approximately 80% con uent MEFs will generally provide between two and four 4-well plates of MEFs depending on the yield of the cells. Protocol 2.5 discusses the inactivation of a 75-cm2 ask of MEFs or a 225-cm2 ask; multiply reagent and medium volumes by 3.

Figure 13-18: A Data Table can display a legend key along with the cross-tabular numeric data normally found only in a PivotTable report.

The last step is to glue a reversal ring onto the front of the 12mm extension tube. For the Canon EOS lens mount, I found that the 52mm 49mm step-down ring fits perfectly (see Figure 12-38). Use the 5-minute general-purpose epoxy on the three tabs of the bayonet lens mount. Then place the step-down ring in the center of the lens mount over the three tabs (see Figure 12-37). In half-an-hour, the epoxy will dry and your automatic lens reversal adapter will be ready for use.

The output is similar to this:

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The neutral gas density in a laboratory vacuum chamber has three components: gas from the thruster, gas from the neutralizer hollow cathode, and the background chamber density. To model the neutral gas density, the gas from ion thrusters can be approximated by isotropic emission from a disk with the diameter of the grid:

slightly from Flash Player 7 to Flash Player 8. Previously, Flash Player 6 disallowed access only between two completely different superdomains, but allowed access between two different subdomains and/or protocols within the same superdomain. For example, Flash Player 6 would allow a Flash application at https://secure.somedomain.com to load and have access to an SWF at http://www.somedomain.com. In Flash Player 7, the domains must match exactly including subdomain and protocol. Although the default security restrictions prevent you from being able to fully load SWF content across domains, subdomains, and protocols, the System.security object enables you to modify some settings that will allow you to grant access to an SWF. The allowDomain() and allowInsecureDomain() methods provide you with the necessary functionality. If you want to specify that another domain and/or subdomain (using the same protocol) should be able to load and use the data from an SWF file, you should use the allowDomain() method. The method should be placed within the first frame of the SWF that you want to load. It allows you to specify a list of parameters indicating locations from which you want the SWF to be accessible. The parameters should be string values, and they can be in the following formats: domainName (for example, www.rightactionscript.com) protocol://domainName (for example, http://www.flashsupport.com) protocol://IPAddress (for example, http://123.456.78.9) Because the Flash Player 7+ model differs slightly from the Flash Player 6 model, the use of the allowDomain() method is slightly different. For example, previously the following code would allow any SWF in the flashsupport.com domain to load the SWF and have access to its data:

The variable name bOriginalUserCBS refers to Calculate Before Save property. The b in front signifies that the variable is a Boolean True or False value. Before moving on to the next topic (using authenticated certificates for digitally signing VBA code), I want to point out that there still is a wide margin of difference between this code and a commercial VBA application. The reason for the wide margin is that commercial applications need to take into a far greater amount of complexity. The completed file of this application is ch10_CalcExperiment_02.xls.

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Panel Align Color Mixer Function Enables you to change the alignment of elements with respect to one another and to the stage. Enables you to mix the colors Red, Green, Blue, and Alpha transparency by percentage values. Also displays Line and Fill palettes, Black and White, No color, and Swap color options. Displays a palette of colors and gradients. Shows the coordinates X and Y, Width and Height dimensions, and Red, Green, Blue, and Alpha (transparency) values. Enables you to duplicate, add, or delete scenes. Enables you to resize, rotate, or skew objects.

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