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The resulting mixture acts as the mobile phase Gradient elution would need to be performed to determine elution conditions for batch ow elution This kind of practice is often used when scaling up chromatography for preparative separation such as in downstream processing of proteins (Section 252) In some cases, combining various gradient steps with isocratic elution may optimize separation of poorly resolved peaks Elution with ionic compounds such as NaCl and KCl in ion exchange chromatography (Section 241) may require high salt concentrations In downstream processing of proteins this salt will need to be removed after chromatography which can be expensive and time consuming An alternative approach is stepwise elution with displacers, that is molecules with a higher af nity for the stationary phase than the target protein of interest These molecules may be of low or of high molecular weight (eg.

The primary sources of knowing are the relationships with the persons close by, those who do the same or a similar job in the same workplace. Yet occasions for the learning and sharing of practice do not necessarily re ect hierarchical relationships. In the workplace, when experience is recognized by those concerned, it takes priority in practice:

heparin and starch derivatives, respectively) and offer the advantage that they induce elution of proteins in highly concentrated peaks of pure material at relatively low displacer molar concentrations (eg 40 mM) Because of the sensitivity of biomolecules such as proteins to their surrounding environment, it is possible to alter.

@AfterReturning( com.mycompany.aspect.SomePointcuts.anythingInSecurityPackage() ) public void someBusinessMethod() { // method body goes here } }

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these problems Some of these services include content segmentation, prefetching, session handoff, and transcoding (Jain et al 2004b; Yoshimura et al 2002) We observe that current CDN implementations lack or do not focus on programmability Many con gurations are made of ine through human intervention and interaction and static con guration information is kept in a con guration le in each surrogate This makes both the maintenance of the CDN and the introduction of new functionalities very dif cult A powerful API will enable CPs to seamlessly and dynamically control how their content is served by CDN networks and will integrate CDN services within their business strategies better For example, consider an on-line merchant, such as Amazoncom, which maintains a complex website to sell products to millions of users The web pages of the website have different characteristics.

Because I sample a lot of shows, my play list can run 100 entries. This can result in a lot of podcasts being stored on my hard drive and added to my portable media device. So I simply create some playlist rules to make it easier to navigate after the podcasts are loaded on my iPod.

Books with elds Title, Author, Year, Pages, Price, and Publisher: It s extremely unlikely that the same author (or two authors with the same name) would publish two books with the same title but Title/Author does not absolutely guarantee uniqueness. You could make the key more unique by adding Year, but it s still not an absolute guarantee. InventoryItems with elds Name, Vendor, Description, and QuantityInStock: It s very unlikely that a particular vendor will have more than one product with the same name (you might not want to do business with such a confused vendor) so Name/Vendor guarantees uniqueness. WeatherReadings with elds Date, Time, Temperature, and Humidity: Unless you take more than one reading at the same time, Date/Time guarantees uniqueness.

The product introduction page has a long lifetime and at any given time multiple versions of the page could coexist in the CDN without much harm Productpricing pages can be updated at any time and multiple versions of the page are undesirable to avoid discrepancy in prices Finally, some sensitive pages, such as those con rming user account information or credit card information, are not cacheable at CDN proxies at all Such differences among various web pages require that the CP have the tools to inform the CSP whether the content should be cached, how long the content can be cached, or when a content update should occur to guarantee synchronization between the cached copy and the original copy Other functions might include purging the outdated or invalided pages from the CSP s local caches or controlling who has the right to access certain content.

7. Give the component a descriptive name to help you identify it for use later. (The Save dialog box looks something like Figure 14-4.) Then click Save.

Such functionalities are mainly supported in two ways The rst is by organizing pages into different directories according to their access characteristics and circulating the directory.

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