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Robustness of the Hash Function Percentage of the Pixel Changed in Data Embedding 17.3.3.3 Time Required to Run the Complete Image/Data Security Assurance 17.3.4 Limitations of the Digital Envelope Method DICOM Security Pro les 17.4.1 Current DICOM Security Pro les 17.4.1.1 Secure Use Pro les 17.4.1.2 Secure Transport Connection Pro les 17.4.1.3 Digital Signature Pro les 17.4.1.4 Media Security Pro les 17.4.2 Some New DICOM Security on the Horizon HIPAA and Its Impacts on PACS Security PACS Security Server and Authority for Assuring Image Authenticity and Integrity 17.6.1 Comparison of Image-Embedded DE Method and DICOM Security 17.6.2 An Image Security System in a PACS Environment Lossless Digital Signature Embedding Methods for Assuring 2-D and 3-D Medical Image Integrity 17.7.1 Lossless Digital Signature Embedding 17.7.2 Procedures and Methods 17.7.3 General LDSE Method Two-dimensional and Three-dimensional Lossless Digital Signature Embedding Method 17.8.1 Two-dimensional LDSERS Algorithm 17.8.1.1 General 2-D Method 17.8.1.2 Signing and Embedding 17.8.1.3 Extracting and Verifying 17.8.2 Three-dimensional LDSERS Algorithm 17.8.2.1 General 3-D LDSE Method 17.8.2.2 Signing and Embedding 17.8.2.3 Extracting and Verifying From a Three-dimensional Volume to Two-dimensional Image(S) 17.9.1 Extract Sectional Images from 3-D Volume with LDSERS 17.9.2 Two-dimensional LDSERS Results 17.9.2.1 Embedded LDSERS Pattern 17.9.2.2 Time Performance of 2-D LDSERS 17.9.3 Three-dimensional LDSERS Results 17.9.3.1 Embedded LDSERS Pattern 17.9.3.2 Time Performance of 3-D LDSERS Application of Two-dimensional and Three-dimensional LDSE in Clinical Image Data Flow

what. However, by selecting appropriate reaction conditions, it may be possible to exclude these side-reactions. For example, N-terminal amino groups react 100-fold more strongly with nondeuterated/pentadeuterated phenyl isocyanate (Figure 10.11) than do lysine -amino groups at pH 8.0. Labelling of C-termini can be achieved by converting COOH groups into methyl esters as shown in Figure 10.12. Methanol containing three hydrogen or deuterium atoms provides the methyl group. As with N-terminal labeling, this reaction will also tag aspartate and glutamate residues. Another widely used C-terminal tagging method that does not label these residues is to introduce 18 O into the C-terminal group formed during tryptic digestion (Figure 10.12). One protein mixture is digested in H2 18 O while the other is digested in H2 O. Either one or two oxygen atoms become incorporated into the C-terminus. It is even possible to achieve this effect by isotope exchange after tryptic digestion. This is done by incubating previously-digested proteins in either H2 O or H2 18 O in the presence of trypsin. 18 O exchanges with 16 O under these conditions. Drawbacks to these C-terminal labeling approaches are the high cost of isotope-labeled water and the possible overlap of isotopic distributions of light and heavy forms of peptides due to incomplete incorporation of 18 O. N- and C-terminal tagging are not mutually

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2. Discuss the mechanism of action of brewer's yeast pyruvate decarboxylase using orbital interaction theory wherever appropriate. For the mechanism, see Zeng, X.; Chung, A.; Haran, M.; Jordan, F., J. Am. Chem. Soc., 1991, 113, 5842. Answer. The mechanism of pyruvate decarboxylase involves use of the cofactor thiamine pyrophosphate TPP (called thiamine diphosphate; TDP in the reference) which contains the thiazolium ring. It is shown in Figure B.1. Each of the ve steps involves some aspect that can be addressed by orbital interaction theory. In step 1, a zwitterionic carbanion is generated. This may alternatively be regarded as a carbene (not zwitterionic at all!) stabilized by two X:-type substituents. Such carbenes are nucleophilic, as we have seen in 7. In step 2, nucleophilic attack occurs at the keto carbonyl group. The principles governing carbonyl reactivity were discussed in 8. Alternatively, an SHMO calculation reveals that pyruvate has a very low LUMO 0X266j j and the largest coe cient of the LUMO is at this carbon atom (0.60 vs. 0.39 at C1). Loss of CO2 in step 3 is facile if the C C bond can adopt a position perpendicular to the plane of the ring, since then overlap with the highly polarized and low LUMO of the aminium group is maximized and electron transfer can occur. The resulting species, shown as a zwitterionic carbanion, may also be viewed as a neutral enolamine, which by SHMO is seen to have a very high HOMO 0X471j j and the largest coe cient on the carbon shown as being protonated by the enzyme in step 4. E2 elimination of acetaldehyde in step 5 is possible if the C C bond and OH bonds can adopt a coplanar arrangement. 3. Allylic acetals rearrange under acid catalysis to produce tetrahydrofurans.

J Comput Phys, 161(1):35 60, 2000 19 P M Gresho and R L Sani Incompressible Flow and the Finite Element Method John Wiley & Sons, Ltd, New York, 1998 20 B T N Gunney, A M Wissink, and D A Hysom Parallel clustering algorithms for structured AMR J Parallel Distrib Comput, 66:1419 1430, 2006 21 W D Henshaw Overture: an object-oriented framework for overlapping grid applications In Proceedings of the 32nd American Institute for Aeronautics Fluid Dynamics, St Louis, MO, June 24 27, 2002 See http://wwwllnlgov/CASC/Overture 22 R D Hornung and S R Kohn Managing application complexity in the SAMRAI object-oriented framework Concurrency Comput Pract Exper, 14:347 368, 2002 23 R D Hornung, A M Wissink, and S R Kohn Managing complex data and geometry in parallel structured AMR applications Eng Comput, 22(3):181 195, 2006 24 A Huber, P Georrgopoulos, R Gilliam, G.

6. Repeat the INSERT command. The Insert dialog box already shows the DOOR block. Click OK. Follow the prompts. You ll probably want to use Zoom Window to zoom into the area of 2 in Figure 18-10.

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